... this particular book more than adequately fills the niche it is intended to cover. [Stefan Surzycki] -- This laboratory manual gives a thorough introduction to modern, cost efficient techniques in Molecular Biology, which can find application in many different fields. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses and workshops or tested in the author's laboratory. MANIPULATIONS WITH DNA: - DNA cloning in plasmids (restriction endonucleases) - Sequencing - Polymerase Chain Reaction (PCR) ISOLATION OF DNA, RNA: Phenol extraction Ethanol precipitation. You may send this item to up to five recipients. WorldCat is the world's largest library catalog, helping you find library materials online. Please try again. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the. This laboratory manual gives a thorough introduction to modern, cost efficient techniques in Molecular Biology, which can find application in many different fields. You're listening to a sample of the Audible audio edition. Identify the workflow of molecular diagnostics. The name field is required. AL10 9AB, UK. Common Techniques in Molecular Biology. The subject field is required. Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, Molecular Biology Techniques: A Classroom Laboratory Manual, Molecular Cloning: A Laboratory Manual (Fourth Edition): Three-volume set. Polymerase Chain Reaction (PCR) is a very common technique used to amplify specific sequences of DNA. The E-mail message field is required. Some features of WorldCat will not be available. This laboratory manual gives a thorough introduction to basic techniques. Please enter the message. http:\/\/purl.oclc.org\/dataset\/WorldCat> ; http:\/\/www.worldcat.org\/title\/-\/oclc\/851823557#PublicationEvent\/berlin_heidelberg_springer_berlin_heidelberg_2000>. 1.1 ENZYMES USED IN MOLECULAR BIOLOGY. Please enter the subject. You can easily create a free account. A Theoretical Consideration -- 11 DNA Transfer and Hybridization -- 12 Northern Transfer and Hybridization -- 13 DNA Cloning. Please select Ok if you would like to proceed with this request anyway. We don’t share your credit card details with third-party sellers, and we don’t sell your information to others. Something went wrong. Learn more ››. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses and workshops or tested in the author's laboratory. PCR is run in a solution with DNA containing the target sequence you wish to amplify, primers, and DNA polymerase. Would you also like to submit a review for this item? Your Web browser is not enabled for JavaScript. After viewing product detail pages, look here to find an easy way to navigate back to pages you are interested in. Copyright © 2001-2020 OCLC. It is absolutely jam packed full of those little titbits of information that friendly Post Docs occasionally feel inclined to share with students. Our lessons will introduce you to ideas that include DNA plasmids, recombinant DNA and restriction enzymes. I liked this book, ... (it) is principally aimed at students ... and the emphasis is very definitely on making techniques accessible to these people. Identify techniques in molecular diagnostics. Please choose whether or not you want other users to be able to see on your profile that this library is a favorite of yours. School of Life Sciences, University of Hertfordshire, Hatfield, Hert fordshire. The distinguishing feature of this manual compared to others is the detailed explanation of the theoretical mechanisms of each step and the discussion of the importance of the techniques. so helpful, I recomend for students who want to deeper your class and lab experience. Gel electrophoresis: Gel electrophoresis is one of the principal tools of molecular biology. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses and workshops or tested in the author\'s laboratory. Predict the DNA sequences based on base-pairing rules. Step-by-step protocols and practical notes are provided. of Oxford, UK in Journal of Cell Science 2000, 113, # Basic Techniques in Molecular Biology\n, 1 General Aspects of DNA Isolation and Purification -- 2 Preparation of Genomic DNA from Animal Cells -- 3 Preparation of Genomic DNA from Plant Cells -- 4 Preparation of Genomic DNA from Bacteria -- 5 Isolation of Plasmid DNA -- 6 Isolation and Purification of RNA -- 7 Isolation of PolyA+ RNA -- 8 Agarose Gel Electrophoresis of DNA -- 9 Preparation of Probes for Hybridization -- 10 Nucleic Acid Hybridization. http:\/\/experiment.worldcat.org\/entity\/work\/data\/14559341#Place\/berlin_heidelberg> ; http:\/\/id.loc.gov\/vocabulary\/countries\/gw> ; http:\/\/dewey.info\/class\/570.28\/e23\/> ; http:\/\/id.worldcat.org\/fast\/832729> ; http:\/\/id.worldcat.org\/fast\/831961> ; http:\/\/experiment.worldcat.org\/entity\/work\/data\/14559341#Topic\/cytology_research_xmethodology> ; http:\/\/id.worldcat.org\/fast\/1065287> ; http:\/\/id.worldcat.org\/fast\/998323> ; http:\/\/id.worldcat.org\/fast\/1014893> ; http:\/\/worldcat.org\/entity\/work\/id\/14559341> ; http:\/\/experiment.worldcat.org\/entity\/work\/data\/14559341#Series\/springer_lab_manual> ; http:\/\/experiment.worldcat.org\/entity\/work\/data\/14559341#Series\/springer_lab_manuals> ; http:\/\/worldcat.org\/entity\/work\/data\/14559341#CreativeWork\/> ; http:\/\/www.worldcat.org\/title\/-\/oclc\/851823557#PublicationEvent\/berlin_heidelberg_springer_berlin_heidelberg_2000> ; http:\/\/experiment.worldcat.org\/entity\/work\/data\/14559341#Agent\/springer_berlin_heidelberg> ; https:\/\/doi.org\/10.1007\/978-3-642-56968-5> ; http:\/\/public.ebookcentral.proquest.com\/choice\/publicfullrecord.aspx?p=3090660> ; http:\/\/dx.doi.org\/10.1007\/978-3-642-56968-5> ; http:\/\/worldcat.org\/isbn\/9783642569685> ; http:\/\/www.worldcat.org\/title\/-\/oclc\/851823557> ; http:\/\/dewey.info\/class\/570.28\/e23\/>, http:\/\/dx.doi.org\/10.1007\/978-3-642-56968-5>, http:\/\/experiment.worldcat.org\/entity\/work\/data\/14559341#Agent\/springer_berlin_heidelberg>, http:\/\/experiment.worldcat.org\/entity\/work\/data\/14559341#Place\/berlin_heidelberg>, http:\/\/experiment.worldcat.org\/entity\/work\/data\/14559341#Series\/springer_lab_manual>. Springer; 2000th edition (April 27, 2000), Reviewed in the United States on November 29, 2014, perfect book!!! Don't have an account? Top subscription boxes – right to your door, © 1996-2020, Amazon.com, Inc. or its affiliates. of Oxford, UK in Journal of Cell Science 2000, 113 Create lists, bibliographies and reviews: Your request to send this item has been completed. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses and workshops or tested in the author's laboratory. This knowledge will enable the users to design their own modifications or to adapt the method to different systems. This knowledge will enable the users to design their own modifications or to adapt the method to different systems. I liked this book, ... (it) is principally aimed at students ... and the emphasis is very definitely on making techniques accessible to these people. Step-by-step protocols and practical notes are provided. of Oxford, UK in Journal of Cell Science 2000, 113. Thus, they are also likely to work in inexperienced hands the first time they are performed. You'll learn about the concept of genetic engineering and study various techniques. Please enter recipient e-mail address(es). BASIC METHODS IN MOLECULAR BIOLOGY MANIPULATIONS WITH DNA: - DNA cloning in plasmids (restriction endonucleases) - Sequencing - Polymerase Chain Reaction (PCR) ISOLATION OF DNA, RNA: Phenol extraction Ethanol precipitation CONVERSION OF mRNA TO cDNA (complementary DNA) Enzyme reverse transcriptase Please try your request again later. http:\/\/www.worldcat.org\/oclc\/851823557> ; http:\/\/experiment.worldcat.org\/entity\/work\/data\/14559341#Series\/springer_lab_manuals>, http:\/\/experiment.worldcat.org\/entity\/work\/data\/14559341#Topic\/cytology_research_xmethodology>. Cover basic concepts and techniques used in molecular biology research labs; Student-tested labs proven successful in a real classroom laboratories; Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an … BASIC METHODS IN MOLECULAR BIOLOGY. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. In addition, each protocol includes an indication of the time and expense involved in its application. CONVERSION OF mRNA TO cDNA (complementary DNA) Enzyme reverse transcriptase. Read on to find out more about what these learning materials cover. BASIC METHODS IN MOLECULAR BIOLOGY.
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